Webinar: New Technique to Monitor DNA Release & Capsid Protein Stability
This dynamic webinar offer insights into characterizing the stability of an engineered adeno-associated virus (AAV) serotype under moderate thermal stress and physiological solution conditions using light scattering measurements. While AAVs are leading vector systems for gene therapy, significant gaps remain in understanding the role of DNA cargo on the degradation propensity and solution stability of capsid proteins.
This webinar highlights experiments that investigated and characterized the effects of thermally-induced perturbations on DNA ejection from a proprietary AAV vector. The experiments were conducted at three temperature setpoints and also determined the absolute molecular weights of full and empty capsid proteins. Interestingly, it was found that the AAV capsid proteins aggregated immediately upon the ejection of DNA, and full capsids with DNA aggregated very slowly. This is an indication of the potential role of DNA in maintaining capsid architecture and preventing capsid protein aggregation.
Defining quantitative and qualitative properties of viral vector systems is critical to ensure maximum efficacy and stability during processing, storage, and parenteral administration. Click below to view this exciting webinar today and gain key insights toward optimizing the efficacy and storage conditions of adeno-associated virus pharmaceuticals.
Meet the Speaker: Prof. Wayne Reed
Prof. Wayne Reed is a co-founder and Scientific Advisor to Yokogawa Fluence Analytics and invented all technologies that contribute to the company’s product portfolio. Prof. Reed is the Murchison-Mallory Chair in Physics and Founding Director of PolyRMC at Tulane University. He holds a Ph.D. in Physics from Clarkson University, an M.S. from the University of Washington, and a B.S. from Rensselaer Polytechnic Institute. He is considered a leading expert in data analysis and characterization methods of synthetic and natural polymers. He has authored more than 150 publications and is listed as inventor on more than 30 issued patents and patent applications.